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Allelic profiles with the three other microsatellites were almost similar in both areas.
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Within the genes, microsatellite regions were almost equally divided over exons and genes (6) and introns (5).
On the other hand, trinucleotides (53.9%) predominated among monomorphic microsatellites, and tri- and pentanucleotides were almost twice as frequent in this group as compared to the polymorphic SSRs.
While sporadic endometrial cancers that have microsatellite instability are almost exclusively of endometrioid type, usually well to moderately differentiated, those related to LS tend to be histologically more diverse also occurring non-endometrioid carcinomas, including serous, clear cell and undifferentiated ones, although the majority is still of endometrioid type [38, 43].
The average distance between two trinucleotide microsatellites (59.9 kb) is almost 10 times that of dinucleotide microsatellites (6.2 kb).
Interestingly, 22.4% of the detected microsatellites were located in coding regions, almost all being short (<14 repetitions) trinucleotide motifs.
This showed that most microsatellites were polymorphic.
Microsatellites are observed in almost all known eukaryotic and prokaryotic genomes, present in both coding and non-coding regions.
Short tandem repeats (microsatellites) were also identified.
Microsatellites were amplified via PCR.
An earlier review of microsatellite inheritance studies found that Mendelian inheritance was almost never rejected for diploid vertebrate species [ 22, 33].
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