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Based on 11 nuclear microsatellites, we identified 86 unique genotypes over the two sampling periods and determined levels of genetic structure.
By testing the various microsatellites, we identified that different microsatellites change the fluorescence intensity of EGFP.
In addition to the perfect microsatellites, we identified 183 compound microsatellites, of which 74.9% were interrupting types and the rest being non-interrupting types (Table 1).
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Using the search terms 'passeriformes [orgn] AND microsatellite' we identified >900 sequences from Genbank.
We also scanned the entire EST set for Sample Sequence Repeats (SSRs, also known as microsatellites), and we identified 5,295 SSRs present in simple formation, within a total of 4,670 (8%) contigs.
In most of the microsatellite pairs we identified, one of the microsatellites was variable in length while the length of the other microsatellite remained unchanged.
Using microsatellite analysis, we identified the distinct parasite populations within these groups of patients.
Within our original microsatellite genotypes, we identified all individuals observed as purebred from the species corresponding to their mitochondrial haplotypes with probabilities > = 0.9 both with STRUCTURE and NEWHYBRIDS.
To understand the relationship between microsatellite length and motif imperfection, we identified microsatellites of different lengths (20, 21, 22, 23 and 24 bp) that either lack mismatch (perfect motifs) or have exactly one mismatch in each locus.
In particular, considering both the sequence and microsatellite datasets, we identify four primary genetic groups: two southern Victorian groups comprising Mt Baw Baw and Mt Buller/Mt Stirling; a Bogong High Plains cluster comprising the Bogong High Plains proper and Mt Buffalo; and a Kosciuszko cluster.
We identified microsatellites and developed primers based on 454 shotgun sequences.
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