Genetic diversity based on microsatellites was high (Table 1, for population based estimates see Table S2).
Primer concentrations were experimentally determined so that the intensity of all microsatellites was high enough to prevent allelic drop-out and allow unambiguous genotyping.
The level of variation found in A. hypogaea using microsatellites was higher than with other markers.
> For both B. rapa and B. oleracea, the frequency of microsatellites was high at/near both ends but low in/near the middle of all the pseudochromosomes (Fig. 3), which likely corresponded to the peri- telomere and centromere, respectively.
The fraction of simple to total perfect microsatellites is highest for mononucleotide and trinucleotide motifs, followed by dinucleotides, hexanucleotides, pentanucleotides, and tetranucleotides.
The cross-species utility of microsatellites is higher than other types of markers.
> -wrap-foot> The mean length of imperfect microsatellites is higher than that of perfect microsatellites in each species (Supplementary Table S3).
Again, the statistical power associated with highly variable markers, such as microsatellites, is high, so statistically significant differences should not always be taken to mean biologically significant differences [ 46].
Unlike other statistics, the power of K to detect microsatellite selection was markedly higher when mutation rate of the targeted microsatellite was high.
Not surprisingly, the predictive value for microsatellite unstable tumours was highest for Amsterdam criteria.
