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A male sheep linkage map comprising 191 microsatellites was generated from a single family of 510 Awassi-Merino backcross progeny.
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This distribution implies that microsatellites are generated in intergenic regions by duplication or transposition.
We propose that the majority of compound microsatellites is generated by tandem duplications of imperfect repeats, mainly at the end of primary microsatellites.
A microsatellite-enriched library was generated based on the protocol of Fischer and Bachmann (1998) with some modifications (Prinz et al., 2009).
A microsatellite-enriched library was generated by Genoscreen genomic platform (Lille, France) for one sample of L. alata from central Gabon (OH2473; Appendix 1) pooled with DNA of three other species, following the methods of Malausa et al. (2011) and Micheneau et al. (2011).
In order to provide a more direct comparison between microsatellites and mtDNA, a phylogenetic tree was generated using 154 unique mtDNA haplotypes from samples for which we also had microsatellite genotypes.
A list of 402 validated SNPs located between the microsatellite markers D20S864 and D20S112 was generated.
A publicly available database was generated for Phytophthora microsatellites, including primers and SSR survey results [ 30].
A collection of 3075 unigenes was generated and candidate microsatellite sequences detected.
Upon every simulated pairing, a haplotype from each parent was generated by random selection of alleles from their microsatellite genotypes.
DNA polymorphism data which was generated by genotyping upland rice accessions from EMBRAPA's germplasm bank using microsatellite markers was used for testing this strategy.
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