Levels of microsatellite-microsatellite LD were comparable to those of microsatellite-SNP LD, though slightly higher (results not shown).
By use of a supervised classification method, we found that using only nine genes the MSI/MSS classification was extremely robust, reaching a sensitivity and specificity that was comparable to microsatellite analysis.
The median age of patients with colitic tumours was less than those with sporadic tumours, but tumour stage, differentiation grade, site and microsatellite instability were comparable between groups (Supplementary Table 2).
Most microsatellites tested for the panel had comparable variation over all breeds, so the selection of microsatellites was based on other standard criteria, such as small product size, robustness of amplification and clarity in scoring.
For example, in the composite map of common wheat 1B chromosome (http://wheat.pw.usda.gov/ggpages/map_summary.html), the genetic distance from the first Glu-B3 marker to the microsatellite Xgwm273.3 is 55 cM, which is comparable to the genetic distance covered from B3-2/B3-3 B3-2/B3-3 B3-2/B3-3tollithesamen in Figure 2B (64.9 cmicrosatellite
In our study, 2500 SNPs were comparable to 250 microsatellites, suggesting that ability to detect genetic losses may depend on the total number of alleles available.
In the above standard population size, the average numbers of alleles per marker locus was 3.83 in monogamy, and 5.31 in polygyny, which would be comparable with the allele number of microsatellite markers in a practical survey.
The rate of redundant SSR-containing clones was found to be comparable (26%) to other studies utilizing microsatellite-enriched genomic libraries in other plant species, e.g., olive tree (Olea europaea L., 16.6%) [ 25], onion (Allium cepa L. 24.3%) [ 23].
Our actual cost of library construction and sequencing, was about US$15,000, is comparable to that charged by private companies for microsatellite enrichment [3].
