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The choice of microsatellites was based on their position in the linkage group, so as to cover the entire map with a minimal set of markers to be used as anchor points.
Most microsatellites tested for the panel had comparable variation over all breeds, so the selection of microsatellites was based on other standard criteria, such as small product size, robustness of amplification and clarity in scoring.
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Densities of microsatellites were based on sequence length excluding segmental duplications and repeats, unless stated otherwise.
The approach taken here for gene-based microsatellite development was based on the targeted identification of SSRs in high-density filter arrayed clones from a large cDNA library.
DNA fingerprinting was based on microsatellite markers for short tandem repeats and karyotyping was determined by Giemsa banding.
This analysis was based on microsatellite variation and mitochondrial haplotypes using Generalised Linear Statistical Modelling in a Model Selection framework.
Pedigree reconstruction was based on microsatellite data analyzed in the maximum-likelihood program CERVUS v. 3.0 (Marshall et al. 1998; also see Kalinowski et al. 2007).
The panel of codominant markers used in fingerprinting was based on microsatellite markers mapping to the A genome of hexaploid wheat.
Unlike previous reports [ 5, 38] the potential interaction between TS expression and microsatellite status with disease recurrence was based on a marginal direct relationship between TS level and outcome for patient with MSI tumors, which did not apply to the MSS subset.
This had already been observed between the map of Chistiakov et al. [ 49] (368 markers) and the first D. labrax linkage map, which was based on 174 microsatellites and spanned 814 cM [ 48].
The calculation was based on autosomal microsatellite genotypes in all 60 individuals.
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