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In the frame of National Program "Aerospatial" The National Institute of Turbomachinery COMOTI, Bucharest, Romania proposes a project of launching with minimum cost of microsatellites using the national territory.
The Spearman's correlation coefficient (rs) was determined to assess potential linkage disequilibrium between CAG and GGN microsatellites, using the native CAG and GGN values, which were not normally distributed.
Individual admixture proportion with the 90% confidence interval based on 23 microsatellites using the structure software.
The set of 3482 unique sequences was searched for microsatellites using the program SPUTNIK.
The set of unique sequences was searched for microsatellites using the SPUTNIK program (http://espressosoftware.com/sputnik/).com/sputnik/
All of the unique sequences were searched for microsatellites using the program Tandem Repeats Finder (ver. 4.00) [ 90].
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PCR primers were constructed based on the genomic sequence of the microsatellites used in the screening of the library.
For the microsatellites used in the final linkage maps the average error rate was 0.34% based on Mendelian inheritance inconsistencies.
As COI (used in our study) is not suited to contemporary gene flow as were the microsatellites used for the G. palpalis gambiensis study [ 51], we recommend the future application of microsatellite data for B. dorsalis s.l.l
These were cleaved using in house PERL scripts and parsed for the identification of the microsatellite markers using the MIcroSAtellite identification (MISA) tool (http://pgrc.ipk-gatersleben.de/misa/) with default parameter setting.
The unique sequences were screened for microsatellites by using the MISA software [ 46].
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