Sentence examples for microsatellites using a from inspiring English sources

We therefore chose to isolate whale shark microsatellites using a repeat oligonucleotide hybridization strategy (a modification of [23]).

Polymorphisms were detected by visual inspection of all microsatellites using a contig viewer program (sean.jar) provided in the SEAN program package.

The assembled data were then queried for the presence of microsatellites using a simple python script using all possible sequences combinations of di-, tri- and tetra-nucleotide repeats, with at least eight perfect repeats.

As negative control plasmids for EGFP expression, mutation resistant (MR) counterpart plasmids (+1 bp OF plasmids) were constructed by changing 1 or 3 nucleotide sequences (A10 to A2CA2GA2CA in TGFBR2 and A8 to A3GA4 in ACVR2) within microsatellites using a Quickchange II site-directed mutagenesis kit (Stratagene, La Jolla, CA), preventing any frameshift mutation (Fig. 1).

The mutational dynamics of DNA slippage have been extensively studied for long microsatellites using a variety of approaches (reviewed in Ellegren 2004).

The EST2uni pipeline identifies di-, tri- and tetra-nucleotide microsatellites using a modified version of Sputnik (C. Abajian, http://espressosoftware.com/sputnik/index.html.html

We examined the distribution of perfect 1-6 bp microsatellites using an optimized detection threshold of 12, 8, 5, 5, 5 and 5 repeats for mono-, di-, tri-, tetra-, penta- and hexa-nucleotide motifs, respectively.

Alsat-1 is a 3-axis stabilised microsatellite, using a pitch momentum wheel and yaw reaction wheel, with dual redundant 3-axis magnetorquers.

We have previously studied mutations within a (CA 13 microsatellite using an enhanced green fluorescent protein (EGFP -based rEGFP -baseday that allows the distinction of reporterion errors assayismathatrepallowsMR) actheity.

We identified 3,723 microsatellites using the software MISA in a non-redundant Physcomitrella expressed sequence tag database comprising more than 37 megabases of nucleotide information.

To examine population structure using nuclear loci we developed primers to amplify microsatellite loci using a modified enriched microsatellite library protocol (Additional file 1).