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These microsatellites represent a major fraction of co-dominant polymorphic markers identified to date in catfish, and should be a valuable resource for genetic mapping to increase linkage map resolution, and for integration of genetic linkage and physical maps.
Microsatellites represent a powerful tool in determining genetic parameters, such as genetic diversity, paternity analysis, gene flow, and genetic drift (Goldstein and Schlotterer, 2001).
When one considers that 26 microsatellites represent a few tiny specks on the canvas of the cancer genome, it becomes apparent that we know little of a complex and evolving picture.
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Therefore, microsatellites represent an insignificant portion of the melon mitochondrial genome and cannot explain its large size.
Every microsatellite represents a block of DNA, a haplotype.
These microsatellites represent 125,363 bp, or 0.7% of the total combined unigene sequence, a density of one SSR every 2.6 kb.
Homopolymers and microsatellites represent unique challenges for whole genome sequencing algorithms designed to call mutations, resulting in a lower efficiency of confidently finding insertion/deletion mutations.
In comparison with the multilocus DNA fingerprinting analyses previously conducted in three-toed woodpeckers [5], application of high-resolution microsatellites would represent a more efficient and straightforward technique for parentage assignment and kinship analyses [9].
Microsatellites represent ideal molecular markers because they have multiple alleles that are highly polymorphic among individuals and loci that are highly abundant and dispersed evenly throughout eukaryotic genomes.
However, several authors have posited that microsatellites represent important targets of selection because high mutation rate allows these loci to accumulate extensive variation that can be drawn upon immediately when environmental conditions change (Kashi et al. 1997; King et al. 1997; Trifonov 2004).
Whether these two mtDNA and microsatellite clusters represent a single species with a very complex population structure (i.e. ancestral polymorphisms maintained by reduced gene flow among subpopulations) or two species with one or more zones of hybridization is unclear from the current data, but may be addressed through the use of coalescent-based models [ 64, 65].
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