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A study to mine SSRs in silico from 22298 EST sequences of eucalypts revealed that primers could be designed for 1244 microsatellites, of which 182 were selected for characterization based on polymorphism status among species (Grattapaglia et al. [2014]).
Additionally, we identified 370 simple sequence repeats (SSRs or microsatellites), of which 69% were trinucleotide repeats, followed by 27% dinucleotide and 4% tetranucleotide repeats (Table 4, Table S6).
The contigs contained 41,470 repeat sequence motifs in coding and/or UTR regions (microsatellites), of which 339 comprised over 7 exact repeat units (Table S1).
There are 83,840 dinucleotide loci with ≥10 repeats (mature microsatellites), of which 35,654 are present within genes.
The contigs contained 46,235 microsatellites, of which 1,608 comprised over 7 repeat units (Additional File Table S3).
A total of 191 microsatellites, of which 104 exhibited sufficient flanking sequences for primer design, and 2197 good quality SNPs were identified for the first time in turbot.
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Further analysis using JoinMap 4.0 allowed mapping of 290 microsatellite markers, of which 161 microsatellites were from BES with significant similarity to zebrafish chromosome 7, and 129 microsatellites were from BES with significant similarity to zebrafish chromosome 13.
For those microsatellites, positions of which were detected well-overlapping with QTL peaks, we did a blast search of the sequences of microsatellites to identify potential candidate genes in these loci.
The primer pairs were designed for fifty-six different microsatellites, 19 of which showed a polymorphism among the genotypes studied.
The search identified 204 genes containing coding sequences with microsatellites, 150 of which were suitable for primer design, but only 103 had nonduplicated primer annealing sites.
This study used diversity evaluations for 100 microsatellites, five of which were selected for characterizing the APA region while the rest were based on the results of Blair et al. [ 34] and were from map locations throughout the genome.
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