Yet, the use of more variable markers such as microsatellites is needed for a full assessment of the nuclear pattern of variation within this species.
In any case, the sufficient discriminating power must be contained in the minimum subset of microsatellite loci needed for accurate individual identification [ 31, 47].
Additional simulations have demonstrated that a set of as many as 500 microsatellites would be needed for much more accurate estimates of R with lower standard deviations (results not presented).
To make significant and timely advances in the genetic improvement of cotton, thousands of portable microsatellite markers are needed for the tetraploid genome of cultivated cottons.
Our results agree with previous suggestions that a large number of microsatellite loci are needed for unequivocal clarification of pedigrees [ 33].
However, for background selection, in which a larger number of markers are needed to provide an overview of the different parental contributions to a progeny's genome, the cost and time needed for microsatellite assays are high or even prohibitive.
For these reasons, profiling of the alleles at several different microsatellite loci is needed to genetically characterize S. cerevisiae isolates.
Ideally, at least one microsatellite from each of the 3307 contigs, possibly more than one microsatellite for large contigs (for orientation), is needed to fully anchor the physical map contigs to the linkage map.
The differentiation index (FST -based phylogenetic tree and Bayesian clustering analysis FST -basedatellite loci suggested the need for 3 MUs: QLI, MSH-QLA, and DXL-XXL-LSH.
One disadvantage of microsatellite markers is they typically need to be developed for each species of interest [22].
To most effectively use microsatellites for divergence estimation, we need to understand their mutation pattern and the factors that affect their mutation rate.
