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This earlier study utilised microsatellites mined from zebra finch EST sequences with very strong similarity to their chicken homologue, but where the repeat region in zebra finch was not necessarily present in its chicken homologue.
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In Oak (Quercus robur) 256 transcribed genes were mapped by exploiting microsatellites mined in their sequences [ 38].
Additional microsatellite loci were mined from publicly available cDNA sequences.
Gene-based microsatellites have been found at a predictable frequency in gene coding regions of many organisms and are often mined from expressed sequence tag (EST) sequences [ 3, 4] although they can also be isolated by various direct laboratory methods from cDNA libraries.
KG and TRS participated in microsatellite mining and data analysis.
The microsatellite mining was performed using the program Msatcommander [ 20].
The 97,565 unigene sequences were downloaded from http://www.genome.clemson.edu/node/273 (presented by the transcriptome sequencing project) (Zhao et al., 2013) and used for subsequent microsatellite mining.
We employed MIcroSAtellite (MISA) http://pgrc.ipk-gatersleben.de/misa/ for microsatellite mining.
Then, Microsatellite identification tool (MISA) (http://pgrc.ipk-gatersleben.de/misa/) was used for microsatellite mining.
Zhao et al. (2013) generated 1,571,764 high-quality reads (assembled into 97,565 unigenes) from vegetative and female buds by transcriptome sequencing using 454 pyrosequencing technology, which provides a large amount of sequence information for microsatellite mining.
Similar observations have also been observed in other genome-wide microsatellite mining studies in monocot and dicot crop plants.
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