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In addition, the linkage to the RH-map of microsatellites makes it suitable for comparison to other QTL studies.
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A comparison of the Techsat lifetime and performance with those of the microsatellites launched before 2000 makes it evident, that among the small satellites with a versatile experimental program, the Techsat is one of the most successful.
The general nature of this protocol makes it suitable for microsatellite library construction in a large variety of plant or animal taxa with a variety of genome sizes.
The map developed in the present study was built with 1,375 markers including 1.268 microsatellites, 105 SNPs and two genes, which makes it more portable to other strains and families.
The multi-locus nature of AFLP thus makes it reliable over the microsatellite procedure as it scans the entire genome.
Furthermore, some of these microsatellite markers made it possible to differentiate the two cell layers L1 and L2 in some clones of both cultivars [ 9, 10].
However, the microsatellite map made it possible to construct in parallel a cytogenetic map, which is not possible with AFLP markers.
An obvious limitation in the development of markers is that mutations in the regions flanking microsatellite loci make it difficult to develop primers that work in a target species.
The availability of the diploid strawberry, F. vesca, genome [ 26] makes it possible to position the microsatellite sequences within the structural context of the strawberry genome.
Microsatellites show a high degree of length polymorphism, which makes it possible to obtain pedigree-level characterization of populations as well as to detect introgression of alleles between species.
Most of the QTL studies that have been performed on sheep were based on sparse microsatellite markers that exhibited large QTL confidence intervals, which makes it very difficult to identify candidate genes for the targeted quantitative trait.
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