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To develop a multiplex polymerase chain reaction (PCR) method for Huntington disease (HD) preimplantation genetic diagnosis (PGD) based on the coamplification of CAG repeats and three different polymorphic microsatellites in a single step of PCR.
Nestling parentage is identified by microsatellites in a study plot at the Hubbard Brook Experimental Forest in New Hampshire.
In an effort to increase the resolution power of our X-chromosome molecular analysis we searched for and identified seven microsatellites in a 118 Kb region containing the Alu insertion polymorphism.
These results strongly support findings from transcriptome-derived microsatellites in a recent study of Baltic herring [ 35].
Very long stretches of microsatellites in a single read, containing up to six microsatellite loci, were commonly observed.
Msatfinder is a Perl script designed to allow the identification and characterisation of microsatellites in a comparative genomic context.
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Thamarus et al. [ 17] found a similar proportion of fully informative markers, 24 in 40 (60%) using a different set of microsatellites in an intraspecific E. globulus pedigree and also did not detect any marker segregating 1 2 1.
Another indel at c. IRGM −308, has been previously described as a tetranucleotide repeat (microsatellite) in a Ghanian population (20).
We have genotyped the D6S1276 Bmicrosatelliteline in a large set of knee OA cases and disease-free controls and found an association with knee OA in women.
Microsatellite genotype data typically takes the form of polymerase chain reaction (PCR) fragment lengths obtained using locus-specific DNA primer pairs to amplify the specific genomic region encompassing a particular microsatellite in a collection of individuals.
MADCE is able to deliver the data required by this software because it can be used to deduce the complete allelic configuration on all homoeologues for a given microsatellite in a given plant, including null alleles and homozygous regions.
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