Three types of association test, GLOBAL, LOCAL and regression-based (REG), are reported for each microsatellite in Table 1 (for an explanation of these tests see Statistical Analysis).
The variability of both microsatellites is described in Table 2.
The distribution and relative abundance of these nonredundant di-, tri-, and tetranucleotide microsatellites is shown in Table 3 and Figure 2. As was seen in the genomic survey, AT, AAT, and AGAT are also the most abundant nonredundant motifs, accounting for 90.30%, 73.52% and 59.48% of di-, tri-, and tetranucleotide motifs respectively (Table 3).
Descriptive data for each microsatellite are shown in Table S1.
Genes that regulate development often contain multiple highly conserved microsatellites in their promoters (table 3).
Consistent with this theory, the perfect microsatellites are found more abundantly than the imperfect microsatellites in each genome (Table 1).
We identified 320 di-, tetra tetra- and penta-nucleotide repeat microsatellites in our UniGenes (Table 5) with minimum repeat thresholds described in the Methods.
Details about the microsatellites identified are provided in Table S6 [see Additional file 6] and include the repeat sequence, the number of times it is repeated, and its location within the host contig sequence.
For each LG with a detected QTL (suggestive or significant), an association analysis was performed between the trait and all microsatellites in that LG (Tables 4, 5 and 6).
Microsatellite locus names, repeat number, repeat unit length, total size, and heterozygosity for each microsatellite can be found in Table S2.
