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Because microsatellites have a high mutation rate and bi-parental inheritance, they can offer more detailed population genetic insight than maternally inherited mitochondrial DNA (mtDNA).
Microsatellites have a high specificity in identification of Leishmania infections owing to their allelic characters [ 37].
All identified microsatellites have a minimum length of 15 bp (see Material and Methods).
Weber [ 10] estimated that, about 10% of the human microsatellites have a composite motif.
37, 38, 46 However, microsatellites have a higher mutation rate than SNPs, which allows detection of recent events.
Microsatellites have a repeat-unit size of 1 6 bp and occupy ∼3% of the human genome (12).
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Wierdl et al. [ 32] suggested microsatellite stability to be related to the length of the stretch of tandem repeats, and postulated that large microsatellites have an increased probability to realign in a misaligned confirmation during replication resulting in a higher mutation rate.
By chance, the selected microsatellites had a wide range of Δ msat values.
The stepwise approach we applied, using 5 instead of 17 microsatellites, had a similar discriminatory power for detecting unrelated infections.
Microsatellites had a high variation in the tea plants [ 33, 37, 38] as well as in other species of Camellia[ 39, 40].
Overall, a total of 2,408 di-, tetra-,etrand penta-, and hexanucleotide microsatellites from 2,225 contigs were identified on the conditions that these microsatellites had a minimum 6, 4, 4, 4, and 4 repeat units and each microsatellite was found in at least 5 contigs.
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