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The resulting data were compared with microsatellites found in genomic DNA sequence (i.e., GSSRs, BSSRs).
Microsatellites found in these libraries may be particularly useful in this regard because they are actually contained within known genes.
These parameters were based on definition of microsatellites found in the literature (details in Buschiazzo and Gemmell 2006, 2009, 2010).
The high frequency of microsatellites found in cucumber genomic sequence compared to other plant species examined (Table 1) was unexpected.
The frequency and type of microsatellites found in the only published Symbiodinium genome [ 29] was higher or similar to that of the studied corals.
Third, the SNPs and microsatellites found in the EST collection could be used to identify genomic regions that may be involved in climatic adaptation.
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Apart from the simple sequence repeats or perfect repeats, the database can be accessed for compound microsatellites (two or more microsatellites being found in close proximity) and microsatellite cluster (compound microsatellites interrupted by few nucleotides).
As expected, we observed a positive trend between the proportion of microsatellites found to be "suspiciously conserved" in each species and sequence divergence, hence phylogenetic distance from human.
However, compound microsatellites, i.e. two or more microsatellites being found in close proximity, have been frequently reported in diverse taxa ranging from humans to plants [ 10, 15- 19].
Microsatellite analysis was carried out using 14 microsatellites loci as described in [ 19] and five polymorphic microsatellites loci found in a cross-species amplification study of T. tambroides using primers from other cyprinids [ 20]: Cyprinus carpio (MFW7 [ 21]), Barbus barbus (Barb37, Barb59, and Barb62 [ 22]), and Barbonymus gonionotus (Bgon13 [ 11]).
In any given species, these microsatellites are found in the same places on the chromosomes of different individuals, but the number of repeats in each place differs from one individual to another.
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