In this study, we constructed a microsatellite enriched library and developed microsatellite loci for future estimating the population genetic diversity based on microsatellite genotyping.
We further provide resources for the design of microsatellite primers for future studies of cnidarian populations.
Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata.
The interspecies transferability made these microsatellite loci useful for future research aiming to reconstruct the phylogeographic patterns and the process of speciation among closely related species.
We developed microsatellites for Manilkara multifida for future conservation genetics studies.
The use of additional cDNA libraries, full length cDNA clones or 3' EST sequencing may be valuable for future microsatellite development, as will analysis with next generation sequencing techniques that can be used to shotgun transcriptomes and reconstruct them bioinformatically.
The availability of sequences from multiple tissues, and potentially scorable SNPs and microsatellites, can form the basis for future studies of different individuals or different species, as has been done in ecological genomic studies of birds (Künstner et al. 2010), cichlid fish (Elmer et al. 2010), and butterflies (Vera et al. 2007).
After optimization and testing for reproducibility and polymorphism, eight microsatellite markers were deemed suitable for future population genetic analysis in the target species.
Amplifiable and transferable microsatellite loci are potentially useful for future studies in investigating intraspecific genetic variation, reconstructing phylogeographic patterns among closely related species, and establishing the standard operating system of molecular identification in Gastrodia.
Conclusions: Four sets of multiplex microsatellite loci can be used for future genetic studies, which includes investigating genetic diversity and structure, and fragmentation and regeneration studies.
