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Microsatellites composed of AT repeats were the most frequent followed by AG and then AC microsatellites (Table 3).
For example, microsatellites composed of the motif AC/TG can absorb negative supercoiling through the formation of Z-DNA, which can displace nucleosomes (Liu et al. 2006; Zhang et al. 2006; Xu et al. 2011) and also prevent the formation of potentially hazardous non-B-DNA structures like slipped-strand DNA (Edwards et al. 2009).
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Microsatellites are composed of tandemly repeated, simple DNA sequence motifs of 1 6 nucleotide bases in length.
Microsatellites are composed of repetitive sequences with 1−10 nucleotides as the repeat unit (reviewed in Bhargava and Fuentes 2010; Gemayel et al. 2010).
Primers Ah51 flank a region that comprises the largest number of repeats (34) and the motif was formed by two nucleotides (A and G) whereas Ah282 flanked a region containing two microsatellites, each composed of six trinucleotide repeats.
Microsatellite DNA is composed of tandem repeats of two nucleotide pairs that are dispersed throughout the genome.
Herein, making use of the extensive T. cruzi genome sequence now available and using the Tandem Repeats Finder software, it was possible to identify and characterise seven new microsatellite loci – six composed of trinucleotide (TcTAC15, TcTAT20, TcAAT8, TcATT14, TcGAG10 and TcCAA10) and one composed of tetranucleotide (TcAAAT6) motifs.
This deletion was within a complex microsatellite repeat mainly composed of AAAG motifs.
RS3 is a complex repetitive region, unrelated to the vole microsatellite discussed above, composed of (CT 4-TT- CT 8- GT 24 where the CT 4-TT- CT 8- GT 24T and GT repeats varies from 16 to 50, yielding sixteen different alleles in the human population [ 8].
REMAP with primer combination Nikita/ CT 9G resulted in a novel 100 bp triticale specific fragment that was not seen in either of the parental genomes, named Mob-110-100T which revealed to be mainly composed of microsatellites, and did not produce any significant alignment against the databases.
SSRs, also called simple tandem repeats (STRs) or microsatellites, are DNA segments composed of tandem repetitions of relatively short motifs.
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