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Microsatellites offer the advantage of reduced ascertainment bias, because the microsatellites are selected to be polymorphic, but the selection process is not conditional on the presence of an individual polymorphism (Haasl and Payseur 2010).
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Following initial analysis that indicated linkage around the ZUBECA1 marker on CFA10, thirty microsatellites were selected from publicly available databases (http://www.vgl.ucdavis.edu/dogset/) in the 25 55Mb region, selecting every 15th microsatellite.
All microsatellites were selected for SSR marker development.
Among those markers, 746 microsatellites were selected and genotyped in the BACE family.
Eighty seven microsatellites were selected according to their sequence length, GC content and functional annotation.
All those microsatellites were selected because they displayed good quality and high polymorphic amplification.
The alignment hits with queried sequences comprising only indels in the microsatellites were selected for further analysis.
A total of 228 microsatellites were selected for genotyping, including a set of 40 heterologous markers (Table S1).
Seventy three microsatellites were selected according to their sequence length, GC content and functional annotation related to abiotic stress category.
BESs harboring at least 50 base pairs (bp) flanking sequences on either side of the microsatellites were selected for PCR primer design.
The fragments containing microsatellites were selected by hybridization with (CT 8- and (GT 8-biotinylated oliGT 8-biotinylatedlloligonucleotideswith Streptavidin MagneSphere Paramagnetic Particles (Promega Corporation, followedg, Wisconsin, USA).
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