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Furthermore, Glaubitz et al. [ 6] estimated in a simulation study that about five times more SNPs than microsatellites are needed to determine pair-wise genetic relationships.
Further analyses using alternative genes and finer scale markers such as microsatellites are needed for further resolution to aid in distinguishing between alternative explanations.
Further fine-scale phylogeographic studies using variable nuclear markers such as microsatellites are needed to clarify species boundaries and give a greater understanding of the processes underpinning the evolution of these taxa across Madagascar.
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Yet, the use of more variable markers such as microsatellites is needed for a full assessment of the nuclear pattern of variation within this species.
Additional microsatellite markers are needed to support conservation studies and breeding programs.
To make significant and timely advances in the genetic improvement of cotton, thousands of portable microsatellite markers are needed for the tetraploid genome of cultivated cottons.
Our results agree with previous suggestions that a large number of microsatellite loci are needed for unequivocal clarification of pedigrees [ 33].
Nevertheless, further studies are needed using more microsatellite loci and larger sample sizes from their sympatric locations in order to reconcile and confirm the hybridization issue.
However, for background selection, in which a larger number of markers are needed to provide an overview of the different parental contributions to a progeny's genome, the cost and time needed for microsatellite assays are high or even prohibitive.
Additional simulations have demonstrated that a set of as many as 500 microsatellites would be needed for much more accurate estimates of R with lower standard deviations (results not presented).
Consequently, more than one microsatellite per cM would be needed in this DG to have any hope of satisfactorily covering the entire genome.
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