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Furthermore, it has been shown that microsatellites are enriched at breakpoints of SDs suggesting the possible role of microsatellite repeats in the genesis of SDs [ 20].
Furthermore, since microsatellites are enriched in both hot IGRs and hot ORFs as defined by the DSB map by Gerton et al., [ 42], and recombination breakpoints mapped on the finest possible scale are concentrated almost entirely in IGRs in yeast [ 55], the relationship between microsatellites and recombination probably is distal to some degree.
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We find that tumors with a microsatellite instable (MSI) phenotype are enriched in genes with repeat instability, and that tumor genomes have significantly more genes with repeat instability compared to healthy tissues.
Though mycobacterial genomes are enriched with microsatellite tracts (Sreenu, Pankaj, Nagaraju and Nagarajaram, manuscript communicated), surprisingly there is yet no report available on the microsatellite mediated phase variation in these bacteria.
This in silico analysis shows that upstream regions of EWS-FLI1 up-regulated genes are enriched for GGAA microsatellites.
We also found that the promoters of genes involved in the development and growth are enriched with highly conserved microsatellites.
We would like to know if a particular category of microsatellites, A (e.g., loci located in coding regions), are enriched in the set of highly conserved loci.
Soil nutrients are enriched.
Methods and Results: Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing.
The PCR was performed on a PTC-100 PCR machine with the following program: 20 cycles of 95°C for 8 s, 68°C for 20 s and 72°C for 3 min. Microsatellites in cDNA were enriched by using biotinylated oligonucleotides and streptavidin-coated magnetic beads.
Next, using functions in the R package 'GOstats' (Falcon and Gentleman 2007), we investigated whether the mature microsatellite-containing genes were enriched for specific GO functional annotations (in comparison to the genes in the remainder of the genome).
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