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Exact(1)
However, "neutral" markers such as microsatellites are designed to reconstruct the evolutionary and demographic history of populations and are theoretically "blind" to the effect of natural and anthropogenic selection that is sometimes very effective and rapid in changing morphological and production traits [ 46].
Similar(59)
Finally, primers to amplify the microsatellites were designed using Primer3 [41].
Eighteen positive library colonies were selected for sequencing, from which 11 microsatellites were designed and tested.
Primers flanking the microsatellites were designed using the PRIMER 3 program [ 45].
Microsatellites were designed and characterized in the African timber forest tree Terminalia superba (Combretaceae).
Primers for BESs containing microsatellites were designed using Primer3 software [ 52].
Specific primers in the flanking regions of the microsatellites were designed using the PRIMER3 software [ 62].
Primers for amplification of selected microsatellites were designed using primer3 [ 47] (See Table 3).
Thirty-nine positive library colonies were selected for sequencing, from which 22 microsatellites were designed and tested for amplification.
Microsatellites were designed and tested with K. tristis from the Thredbo 2 population and details of the design methodology are given in [ 68].
A total of 6471 primer pairs bordering microsatellites were designed, and 96 pairs were selected for PCR amplification trials using eight individuals representing the seven taxa collected from a broad range of the Japanese archipelago (Appendix 1).
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