Primer sequences for the microsatellite markers and map positions were obtained from the UniSTS database of the National Center for Biotechnology Information (Supplementary Table 4); primer sequences for the SNPs were designed using Primer3 software (v0.4.0).
LLC, FF, GP, LJ, ZZY and LG are involved in screening the library with randomly selected microsatellites and genes, mapping of markers to the linkage map.
We describe the first genetic linkage map of Siberian jay constructed using 117 microsatellites and a mapping pedigree of 349 animals representing five families from a natural population breeding in western Finland from the years 1975 to 2006.
In total, this consisted of 4350 individual markers, including: 4089 DArT, 253 microsatellites and eight mapped genes.
Although a long time effort has been made, the kelp microsatellites isolated and mapped were extremely scarce [ 23, 66, 67, 69].
This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus.
To facilitate the mapping of quantitative trait loci (QTL) for important traits and comparative genomics studies, we have constructed a consensus linkage map of Mozambique tilapia and red tilapia using microsatellites, and conducted comparative mapping.
This report describes the development of a novel set of 230 EMBRA microsatellites, the construction of the first comprehensive microsatellite-based consensus linkage map for Eucalyptus and the consolidation of existing linkage information for other microsatellites and candidate genes mapped in other species of the genus.
One conceivable explanation for the differences between the microsatellite and SNP maps is that the proportion of missing data was three times greater for microsatellties (15%) than SNPs (5%).
It could be argued that because the SNP map was used to help assemble the zebra finch genome its errors could have been incorporated into the physical assembly, thereby making the assembly an inappropriate reference against which to compare the microsatellite and SNP maps.
