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The detection limit of the method on a microplate scale was 10 μM PA.
These models provide a rigorous engineering basis for more reproducible and rational selection of operating conditions for microplate scale cell cultivation.
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The amount of fluorogenic peptide probe sourced from either of two (thrombin or HIV-1 protease) commercial biochemical activity assay kits intended for ∼100 microplate-scale reactions is sufficient for 15 droplet-scale screening campaigns, performing ∼50 million total assay reactions.
This non-classical model is capable of incorporating the microplate model with the length scale parameter, geometric nonlinearity, transverse shear deformation and rotary inertia.
according to the manufacturer's protocols, but scaled down to microplate volumes.
The assay was performed generally according to the manufacturer's protocol but scaled down to microplate format.
This microplate model incorporates the material length scale parameter that can capture the size effect in FGMs.
The results show that the size effect on the bending, buckling and vibration characteristics is significant when the ratio of the microplate thickness to the material length scale parameter is smaller than 10.
The phosphodiesterase assay was scaled down to a microplate reader format (Biochrom Asys Expert 96) but essentially performed as described previously (Hynie et al., 1975).
Free fatty acids were determined by an enzymatic colorimetric method of the Wako nonesterified fatty acid (NEFA) C test kit (Wako Chemicals GmbH, Neuss, Germany), scaled down to a microplate format.
It is found that the size effect is significant when the thickness of microplate is close to the material length scale parameter.
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