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Microplate reads were carried out every 10 min using a 595 nm absorbance filter.
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Finally microplates were washed, the reaction developed, and microplates read at 450 nm.
The cells were washed twice again and 50 µl LLB per well were added, and plates will be ready for microplate reading or capturing images with fluorescent microscopy.
For microglia, 5×104 cells per well (in 96-well plate for immunofluorescence and microplate reading), or 2×105 cells per well (in 24-well plate for biochemistry) were seeded for 1 week in Dulbecco's modified eagle medium (DMEM) supplemented with heat-inactivated 10% fetal bovine serum (FBS), 50 µg/ml penicillin/streptomycin (all from Invitrogen) and M-CSF.
UIC was analysed using an adaptation of the Sandell-Kolthoff method using an ammonium persulfate digestion and microplate reading.
Taken together, our results show that fluorimetric microplate reading tests using propidium iodide and Sytox were efficient for larvae viability analysis after treatment with ivermectin and albendazole sulfoxide.
The OBS microplate was read on a FLUOstar OPTIMA fluorescence microplate reader (BMG Labtech, Aylesbury, UK) at 485 nm excitation and 620 nm emission wavelengths.
The 96-well microplates were read using ThermoLabsystems OpsysMR microplate spectrometer.
Finally, the microplates were read on a microplate reader (Bio-Rad Laboratories) at 570 nm.
The solutions of recovered dye were transferred to a 96-well microplate and read at 513 nm on a SpectraMax 190 microplate reader (Molecular Devices, Sunnyvale, CA).
The plates were well shaken for 5 min, and an OPSYS microplate reader read the absorbance at 570 nm from DYNEX Technologies Inc. (Chantilly, VA).
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