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The material properties of FG microplate change continuously along thickness direction according to simple power law.
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The color changes developed over time on the right side of the microplate indicate changes in cell viability, which was determined using the MTS protocol.
NF-D1, various concentrations of recombinant p50, and NF-D2 were mixed in a half-area 96-well microplate and changes in fluorescence were measured.
Culture media in the microplates was changed every 3 days.
Our results with the AFM stiffness clamp are consistent with a recent study by Mitrossilis et al. that used a custom-built parallel microplate system to change the stiffness experienced by a single myoblast cell and found that traction rate was higher for larger stiffnesses and did not depend on absolute force [22].
Different amounts of purified enzyme and substrate in the presence of the cofactor NAD+ (Sigma) (400 µM in 100 mM Tris/HCl pH 9, 25 °C) were incubated in a SpectraMax M5/M5e Microplate Reader and the change in nucleotide cofactor absorbance (A340 nm) was measured.
Removal of N by progressive depletion cannot be controlled in microplates, and centrifugations to change the medium are difficult or impossible to achieve depending on the strain with or without flagella.
Each plant sample was extracted twice and enzyme activity was tested by monitoring absorbance changes on a microplate reader (BioTek Synergy HT, BioTek Instruments, Winooski, VT) at A340.
Bacterial growth was determined by observing the color change of INT in the microplate wells.
The minimal biofilm eradication concentration (MBEC), defined as the lowest concentration of an antimicrobial agent required to eradicate biofilm, was determined by observing the color change of INT in the microplate wells as per MIC determination method.
The minimum biofilm inhibitory concentration (MBIC), defined as the lowest concentration of an antimicrobial agent required to inhibit the formation of biofilms was determined by observing the color change of INT in the microplate wells as described above.
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