Sentence examples for microplate bioassay from inspiring English sources
Statistical analysis showed equivalency between agar diffusion microbiological assay and rapid colorimetric microplate bioassay.
The aim of this work is to develop, optimize and validate a rapid colorimetric microplate bioassay for the potency of neomycin in pharmaceutical drug products.
In addition, microplate bioassay had advantages concerning the sensitivity of response, time of incubation, and amount of culture medium and solutions required.
For analysis of bioactive mixtures, this setup is a powerful and versatile alternative to traditional fractionation processes followed by microplate bioassays.
Conventionally, when dealing with bioaffinity assessment of mixtures, they are first fractionated [ 1], the collected fractions are evaporated, and microplate bioassays are used to detect the bioactive fractions, eventually in parallel with compound identification by mass spectrometry (MS) and/or nuclear magnetic resonance (NMR) spectroscopy [ 2– 7].
Antimycobacterial bioassay was performed using the Resazurin microplate assay (REMA) [ 15].
Urine cAMP was normalised to creatinine concentrations, which was measured using a commercial microplate Jaffè reaction kit (Quanti Chrom Creatinine Assay, BioAssay Systems, Hayward, CA, USA).
Absorbance of bioassay solutions was read on an xMark microplate spectrophotometer.
The optimized assay conditions for the FP microplate reader format were transferred to an on-line bioassay format in FIA mode.
Fluorescence bioassay data were collected with a multi detection microplate reader SynergyTM HT (Bio-Tek® Instruments Inc., Winooski, Vermont, USA), with 360 nm excitation and 460 nm emission filters, and analyzed using KC4 software (Bio-Tek®Instruments) and a Microsoft Windows XP.
TSA concentration in the serum was measured on the Microplate Fluorescence Reader FL600 (Bio-Tek, USA) according to the enzymatic method (EnzyChrom Sialic Acid Assay Kit, BioAssay System, Hayward, USA) using the colorimetric procedure.
