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The use of ultraviolet (UV) light for water treatment disinfection has become increasingly popular due to its ability to inactivate chlorine-resistant microorganisms without the production of known disinfection by-products.

Metagenomics with deep sequencing is a recent advancement of a molecular technique that enables genomic analysis of microorganisms without the need to isolate and culture pathogens.

PNA probes, which are synthetic oligomers mimicking the DNA or RNA structure, allow the detection of microorganisms without the need for an amplification step and are thus less likely to be affected by contamination [ 40].

The experimental approach employed in this study is a valid tool to unveil biological events triggered directly by the presence of intracellular microorganisms, without the mediation of soluble factors.

Mannan-binding lectin (MBL) is the only known collectin known to activate the C system and binds multivalently to terminal mannose, N-acetylglucosamine, glucose and fucose on yeast cells and Gram-negative bacteria, thereby promoting phagocytosis of the microorganisms without the involvement of antibodies.

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Also, to avoid possible infections, the ability of hypochloric acid, a disinfectant, to suppress the relevant microorganisms without changing the life style and behavior of leeches in terms of sucking function, is investigated.

Anti-infectives are substances that in small doses can inhibit the growth or the survival of microorganisms without affecting the host (Foye et al. 1995; Korolkovas 1976).

Second, probiotics, known widely as beneficial bacteria and yeasts, assist in the restoration of normal levels of beneficial microorganisms without destroying the bacterial communities of the GI tract.

The optimum concentration of hypochloric acid which eliminated microorganisms without affecting the viability and sucking function of the leeches were determined by dilution of hypochloric acid to 100, 50, 25, 12.5, 6.25 ppm concentrations in different groups of 25 leeches.

Thus, sulfa drugs can inhibit the growth of invading microorganisms without harming the host.

Research should be directed towards the development of ultra-fast diagnostic techniques that can immediately predict causative microorganism, without the need of specimen processing and also detect multirresistance mechanisms to avoid inadequate initial antibiotic treatment.

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