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All microorganisms were stocked in appropriate conditions and regenerated twice before using.
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All microorganisms were stored at −80°C as a stock and maintained at 4°C on Mueller-Hinton Agar (Merck, Germany).
All microorganisms were stored as frozen skim milk stocks at -70°C and were passaged three times before use in any testing.
Planktonic microorganisms were segregated into latitudinal belts.
No microorganisms were identified.
He has published numerous scientific papers where genetically engineered microorganisms were used to produce high value therapeutic proteins.
‡The microorganisms were misidentified.
All identified microorganisms were recorded.
Residues and by-products from plants, animals, and microorganisms are a valuable stock material to be used in a wide range of sectors, including bioenergy, fertilizers, food, feed, biomaterials (textiles, adhesives), or other fields.
To determine the survival rate of microorganisms after freezing, one week after stocks were prepared, 3 aliquots for each strain were defrosted and the number of microorganisms was assessed by plating 10-fold dilutions of the bacterial suspension, in duplicate, on Middlebrook 7H11 agar (BD Science).
"Microorganisms are not interested in it," he said.
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