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Exact(8)
Calibration curves for each of the microorganisms were prepared by performing serial tenfold dilutions (up to 10− 5) of bacterial and yeast suspensions of known optical density.
The inocula of microorganisms were prepared from a 17 h broth culture and the suspensions were adjusted to 0.5 Mc Farland turbidity.
The inoculums of all microorganisms were prepared from an overnight culture grown aerobically in Mueller Hinton (MH) broth at 37 °C.
Two replicas of the respective microorganisms were prepared by spreading 100 μl of revived culture on the nutrient agar plate (composition (g/l) peptone, yeast extract, sodium chloride, D glucose and agar agar) with the help of a spreader.
Inocula of 0.5 MacFarland (1 × 108 CFU/ml) microorganisms were prepared and inoculated onto a Mueller-Hinton agar (MHA) plate.
For the control, Eppendorf tubes with 1.5 mL of solution containing 1350 μL and 150 μL CSD suspension of microorganisms were prepared.
Similar(52)
A standardized suspension of microorganisms was prepared for use with a 0.5 tube on the McFarland scale.
All microorganism cultures were prepared from their respected slants.
Microorganism suspensions were prepared from 24 hours cultures of bacteria in Tryptic Soy Broth (TSB) by adjusting 0.5 McFarland turbidity standard (10 CFU/ml).
Inocula were prepared by transferring microorganisms from stock cultures onto tryptic soy agar plates containing neutralizer which were incubated for 24 – 48 h at 37 ± 2°C.
To determine the survival rate of microorganisms after freezing, one week after stocks were prepared, 3 aliquots for each strain were defrosted and the number of microorganisms was assessed by plating 10-fold dilutions of the bacterial suspension, in duplicate, on Middlebrook 7H11 agar (BD Science).
More suggestions(15)
charities were prepared
agents were prepared
charges were prepared
plants were prepared
organizations were prepared
agencies were prepared
bacteria were prepared
microorganisms were detected
microorganisms were isolated
microorganisms were found
microorganisms were cultured
microorganisms were identified
microorganisms were spiked
microorganisms were classified
microorganisms were defined
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