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However, not all microorganisms store TAG from wastewater in an appropriate way for biodiesel production.
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All microorganisms were stored at −80°C as a stock and maintained at 4°C on Mueller-Hinton Agar (Merck, Germany).
The pure cultures of microorganisms were stored at cooling conditions until the start of the plate tests.
After the incubation, the tubes containing the microorganisms were stored at 4.0°C ± 0.5°C.
The microorganisms were stored at −70°C in Sabouraud Dextrose Broth (SDB, Merck® - C. albicans) and Mueller-Hinton Broth (Difco® - bacteria) with 15% glycerol.
All microorganisms were stored as frozen skim milk stocks at -70°C and were passaged three times before use in any testing.
Within biological wastewater treatment (BWWT) systems, specialized microorganisms assimilate and store lipids anaerobically.
Polyhydroxyalkanoates, the natural polyester that many microorganisms accumulate to store carbon and reducing equivalents, have been considered as a future alternative of traditional plastic due to their special properties.
Environmental waters used to prepare the photo degradation experimental solutions were filtered twice using a 0.2-micron 47 mm glass fiber filter to remove any particles and microorganisms and then stored in the dark at <4°C until experiment was performed, typically within a week.
For patients who consent, we will store recovered microorganisms for use in potential future infection control studies.
Sulfur is an important element for microbial life present in deep-sea environments and is metabolized by a wide variety of microorganisms [27], which transiently store sulfur in intracellular vesicles.
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