The protocols and principles developed in this study provided valuable references for genome engineering in other microorganisms lacking developed genetic engineering tools.
The majority of microorganisms lack combination of all properties.
Lacking a microorganism's capacity to multiply, a drug would soon be diluted to the point of ineffectiveness as it was passed in blood from victim to victim.
The elements located in this region include genes encoding a periplasmic component of a transport system (nrtA), a putative nitrate transporter (narK), the large and small subunits of nitrite reductase (nirB and nirD) and nitrate reductase (narB), plus a hypothetical protein conserved among several microorganisms that lacks an assigned function.
B. subtilis served as negative control for the prediction approach, since as a Gram-positive microorganism it lacks an outer membrane, and therefore all predicted outer membrane proteins can be considered false positives.
We anticipate that systematic surveys of ancient cocoons may open a unique window into the evolutionary history of a range of soft-bodied microorganisms that otherwise lack a fossil record.
SERPIN molecules within the midgut of the sand fly may serve to counteract damaging proteases produced by microorganisms; however LuloSRPN lacks a predicted signal peptide sequence and thus may serve an intracellular housekeeping function.
The reference database, typically containing a gene catalog, a collection of contigs, or genomes, is often incomplete because most microorganisms encountered in metagenomes lack a sequenced reference genome.
Biochemical as well as cultural methods for the detection of bacteria and coliform have some drawbacks such as long incubation period, contamination with other microorganisms, lack of precision and required sensitivity and poor identification of VBNC (Viable but not culturable bacteria) bacteria.
The observed reductions in bacterial loads could be due to settling of organisms to the bottom of storage containers or die-off of these organisms caused by predation by other microorganisms, lack of nutrients, or other factors contributing to inhospitable conditions in the container.
