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Microorganisms embedded in Precambrian chert attest to the chemical integrity and physical stability of chert [64, 67, 68].
The pathogenesis of device-associated infections with staphylococci is mainly characterized by the pathogens ability to colonize the surfaces of the implanted medical device by the formation of a three-dimensional structure of microorganisms embedded in a thick extracellular matrix composed of polysaccharides, proteins, extracellular DNA, and host factors, known as biofilm [3], [4], [5].
The ultrastructural examination of this deposit revealed microorganisms embedded in matrix.
To eradicate microorganisms embedded in biofilm, antibiotic concentrations would have to be 100-fold to 1,000-fold 1,000-foldred thatill frequiredtong organisms [ 97, 98].
Theliade et al. demonstrated that BS is a deposit consisting of microorganisms embedded in an intermicrobial substance with a tendency to calcification.
In the present paper, we have studied in detail, by SEM-BSE (scanning electron microscopy in backscattered electron mode) associated to EDS (energy dispersive X-ray spectrometry) microanalysis, the degree of biological conservation and the mechanism/s involved in the preservation of the eukaryotic microorganisms embedded in this ancient amber.
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Once microorganisms are strongly attached to the membrane, and after the EPS production is activated, the microorganisms eventually become embedded in the hydrated matrix and are immobilized.
A biofilm is a biologically active matrix of cells, a microbial community, or a functional consortium of microorganisms that is embedded in exopolymeric substances produced by them and associated with or attached to a solid surface, such as equipment within meat processing environments.
Bacterial pathogens, including L. monocytogenes, are known to form structured populations of microorganisms, adhered and embedded in an extracellular matrix consisting mainly of exopolysaccharides (biofilms) [35].
The scanning electron micrographs (SEM) showed typical coccoid-shaped cells as the dominant microorganisms on the granule surface embedded in an extracellular polysaccharide (EPS) matrix), very similar to those reported by others [ 53, 93, 94]. Figure 3(b) showed the typical crescent-shaped ANAMMOX cells obtained via transmission electron micrograph of chemically fixed ANAMMOX cells [ 95].
According to Costerton et al. [1], a biofilm is "a functional consortium of microorganisms attached to a surface and is embedded in the extracellular polymeric substances (EPS) produced by the microorganisms".
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