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Majority of these communal microorganisms are difficult to grow in vitro.
Results obtained by our group and by Hayette et al. [ 26] may suggest that those microorganisms are difficult to isolate in culture or they may be unable to grow and survive in the hospital water supply system.
The bacteria cannot enter the replicative cycle when the pH is between 4.0 and 6.0, and the microorganisms are difficult to eradicate when they assume the phenotypically resistant state [ 15, 16].
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The versatility of molecular manipulations and protein expression in yeast allows for the rapid construction of comprehensive protein expression arrays to explore the function of pathogen-specific gene products from microorganisms that are difficult to genetically manipulate, grow in culture or too dangerous for routine analysis in the laboratory.
NGS has the potential to replace several existing tests performed on the same isolate, notably identification of antibiotic-resistance mechanisms, virulence determinants and genotype, in particular for microorganisms that are difficult to grow.
Parallel testing for Chlamydia spp. was included to explore the frequency of co-infections with 2 microorganisms that are difficult to propagate and that can cause disease patterns that may be clinically indistinguishable.
Body fossils and fossilized colonies of microorganisms exist but are difficult (and controversial) to observe and verify, as are even more subtle features, such as potential microbial corrosion phenomena (Furnes et al., 2004; Westall et al., 2006a, 2006b, 2011a; Foucher and Westall, 2009; Grosch and McLoughlin, 2014).
Technical scale (≥5 l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium.
Phagotrophic microorganisms can be difficult to study in the laboratory because of their requirement for other organisms as food.
Microorganisms belonging to the B. cepacia complex are difficult to identify by conventional biochemical tests and commercial systems (8 ).
Microorganisms, by definition, are tiny, and as such are difficult to isolate, grow and study.
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