A reverse-phase C18 column (Phenomenex 250 mm × 4.6 mm, 5 micron) was used at a flow rate of 1.0 mL/min.
Alumina particles of size 50 microns were used as the erodent.
Advanced PCB techniques, offering typical feature size of the order of a few microns, are used to construct the detectors, which show excellent spatial resolution and fast charge collection.
An annulus width of 0.3 microns was used, and the density was calculated by dividing the number of points within an annulus by the area of that annulus.
We assume a well-mixed microenvironment, hence a volume of 100 microns is used in the numerical simulations.
Actual commercial fiber, sized to rat-respirable dimension, having an average fiber diameter of 1 micron and an average length between 15 and 25 microns, was used in the experiments.
A step size of 25 microns is used to sample these baseline gradients in relatively deep penetrating vessels, imaged with sufficient signal to background to nearly 400 µm (Media 3).
Four micron sections were used for the tissue arrays.
This is a typical case study of RO plant installation where physical methods of pretreatment such as sand and micron cartridge filters were used; no chemical pretreatment of feed was employed for long-term brackish water desalination study.
Four micron tissue sections were used for immunohistochemical staining using primary Abs to the following antigens: human Activin A (clone E4, dilution 1∶50, Serotec, Oxford, UK), human Langerin (clone 12D6, 1∶200, Vector Laboratories, Burlingame, CA), human Factor VIII-related antigen (rabbit polyclonal, 1∶100, Neomarkers, Westnghouse, CA).
