Scanning resolution was 100 microns and the photomultiplier tube was set to 550 V.
Confocal images were obtained with a Leica SPE upright confocal microscope with 63×N.A. = 1.4 objective and the thickness of optical slides was set to 0.3 microns.
The defocus level was set at −6.0 microns.
Slice thickness was set at 250 microns using a tissue slice thickness gauge (Alabama Research and Development) and slices were cut using a reciprocating blade at 30 rpm.
The pellet was set in resin, and micron-sized slices were cut.
For the simulations presented here, the mesendoderm explant length was set to 71percentt of the total length of the simulation area, or 309 microns, while the width was set to 21percentt, or 85 microns.
The configuration was set as the one in 24th April 1991 (one year after the deployment) for f555w filter, object spectrum K4V and a null secondary despace (0.0 microns).
Using the giant water vapour maser at 22 GHz in Orion as a source, MORRIS, et al [17] achieved a measurement accuracy of 30 microns and were set the surface of the IRAM 30 m millimeter telescope to an accuracy of better than 100 microns.
Flow cytometry gates were set using 2 micron beads (Figure 1A, inset).
To confirm that the microvesicles were the correct size, flow cytometry gates were set using 2 micron bead standards (BD Biosciences).
