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Representative photographs of the transilluminated stained sections on a Zeiss stage micrometer were collected with a Canon EOS 1000D digital camera.
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Ten-micrometer cryostat sections were collected directly onto slides with a permanent positively charged surface (Superfrost/Plus Slides, Cat # 12-550-15; Fisher ScieNHific, Hampton, NH, USA).
Five-micrometer thick frozen sections were collected on Superfrost Plus glass slides (VWR international; Leuven, Belgium), dried overnight at room temperature (RT) and subsequently fixed in acetone for 10 min.
For the analysis of the distribution of bacteria, approximately 400 × 400 × 100 μm volumes were collected with resolution of 0.8 micrometers in x and y and 5 μm in z.
3D image stacks were collected for each nucleus at 0.2 micrometer Z-spacing.
From the tracks, the following data were collected for each peroxisome: mean speed (micrometers/second), distance traveled (micrometers) and displacement distance (distance between start and finish locations, micrometers).
These fibers — less than one micrometer in diameter — can be collected to form a large-scale wound dressing or bandage.
[32] After purging, a 50 milliliter (ml) filtered [0.45 micrometer (μm) nominal pore size] sample was collected.
Consequently, due to the excitation power quadratic dependence of the nonlinear effect, depth from which 2PEF and SHG light is collected does not exceed a few tens micrometers.
The supernatant was collected as the crude venom and then filtered through a 0.22 micrometer cellulose acetate filter.
In this way the charged nanoparticles can be collected on a solid surface forming uniform coatings with thicknesses from tens of nanometers to several micrometers thick.
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