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This is because we did not fix the cantilever at only one surface site, but rather scanned it over a micrometer area, which is much larger than the etched depth, as schematically depicted in Figure 4b.
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The acquired video microscopic image was divided into 512 × 512 pixels, and each pixel was expressed in square micrometers (area) after calibration with the current magnification.
Furthermore, this approach enables nanoscale resolution of microbial surface throughout a range of tens of micrometer square area.
This poses a challenge for a growing number of future nanopore sensing applications, such as high throughput DNA sequencing or protein detection, which benefit from the ability to simultaneously probe many pores fabricated in a small (micrometer scale) area.
In addition, the size of the areas with myelin or axonal damage was measured using the same 1-cm ocular grid at ×10 magnification and expressed as micrometer squared areas of myelin or axonal damage per 10 fields.
However, we found the fact that scanning with full power caused bleaching of PBsomes not only in the focusing plane, but also perpendicularly, in a few-micrometer flanking areas through the cell (data not shown).
The thickness of the tendon strip was measured with an area micrometer.
Moreover, we measured the thickness of each tendon strip 3 times using an area micrometer and the average of these 3 values was used for further analysis.
Extended luminal surface area, micrometer-scale vessel caliber, and low flow rate favor interaction of particles with endothelium in this vascular segment.
In the modern research world, electrospinning is one of the most frequently used techniques for the preparation of nonwoven fibrous materials with an ultrafine diameter (ranging from few nanometers to several hundred nanometers or even micrometers), high surface area per unit mass, and small interfibrous pore size [ 1– 3].
d Location map of the OTFT array and contact area with micrometer rod.
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