Sentence examples for microgram was treated from inspiring English sources

One microgram was treated with DNase I (Invitrogen, 18068-015), reverse transcribed (Invitrogen, 18080-040), and fifty micrograms run in a standard PCR reaction.

After isolation with the Ambion RNaqueous kit (AM1912, one microgram RNA was treated with DNAse, (Invitrogen, 18068-015), then reverse transcribed with Superscript III First Strand kit (Invitrogen, 18080-040).

One microgram DNA was treated with 5U Degradase at 37 °C for 1 h, in a final volume of 25 μL and subsequently inactivated by adding 175 μL of 0.1% formic acid.

Two micrograms DNA was treated with sodium bisulfite using the Epitect kit (QIAGEN GmbH), PCR amplified, cloned into E.Coli and sequenced.

One microgram of RNA was treated with RNase free DNase (Promega, USA) and then reverse-transcribed using the iScript cDNA Synthesis Kit (Bio-Rad, USA) according to the recommendations of the manufacturers.

One microgram of RNA was treated with DNase to eliminate any contaminating genomic DNA and subsequently reverse transcribed.

One microgram of RNA was treated with RNase-free DNase (Ambion, 1 unit) for 0.5 h to eliminate any genomic DNA contamination.

One microgram of DNA was treated with bisulfite using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Irvine, NY, USA) according to the manufacturer's instructions.

One microgram of DNA was treated with sodium bisulphite to convert cytosine to uracil using the EpiTect® Bisulfite kit (QIAGEN, Valencia, CA, USA) according to the manufacturer's recommendations.

Forty micrograms of RNA was treated for DNA contamination using RNase-free DNase set (Qiagen).

One microgram of total RNA was treated with reverse transcriptase (Superscript II; Invitrogen) for 1 h.