Sentence examples for microgram samples of from inspiring English sources

Ten microgram samples of fragmented cRNA were hybridized to GeneChips for 16 hours at 45°C.

For Western blot analysis of CXCR4, 30 microgram samples of whole cell lysate were separated by electrophoresis on 10 20% SDS polyacrylamide gels and transferred to PVDF membrane by electroblotting.

For Western blot analysis of CXCR4, 40 microgram samples of nuclear extracts or cytoplasmic fractions were separated by electrophoresis on 10 20% SDS polyacrylamide gels and transferred to PVDF membranes by electroblotting as described previously [ 3].

Fifteen-microgram samples of the resulting supernatant were subjected to SDS polyacrylamide gel electrophoresis.

Each sample was dropped into a Mo crucible and was heated stepwise at 400, 650, and 1800 °C to extract noble gases in a "pot-pie" furnace designed for noble gas analysis of microgram samples.

Ten microgram of sample was applied in each well of 20%% polyacrylamide gel and was run with running buffer of 50 mM Tris, 384 mM glycine, 2 % SDS.

This work described a quantitatively robust label-free phosphoproteomic workflow capable of analyzing relatively small amounts (100s of micrograms) of sample directly from any biological matrix, including human tissue and biological fluids.

The Water Authority shows pesticide levels as high as 91 micrograms a liter in samples of drinking water.

One microgram of sample was lysed in Laemmli buffer, electrophoresed, transferred onto membranes and incubated in 0.2% milk/TBS-Tween solution containing a serum raised against a peptide in the cytoplasmic domain of CD32a (CT10; Ibarrola et al, 1997).

Requiring only sub-micrograms of sample DNA, the power of AluScan as a discovery tool for genetic variations was demonstrated by the identification of 357 instances of loss of heterozygosity, 341 somatic indels, 274 somatic SNVs, and seven potential somatic SNV hotspots between control and glioma DNA.

Five micrograms of the 20 RNA samples of each inbred line were pooled and 100 μg total RNA per inbred line was sent for cDNA synthesis to vertis Biotechnology AG (Freising, Germany).