Sentence examples for microgram of total from inspiring English sources

One microgram of total RNA was subjected to RT reaction using Bestar qPCR RT Kit (DBI Bioscience, Ludwigshafen, Germany).

Fifty microgram of total protein extracts were loaded for SDS-PAGE, and then western blotting was performed.

One microgram of total RNA was reverse-transcribed to cDNA following the manufacturer's instructions.

One microgram of total RNA was treated with reverse transcriptase (Superscript II; Invitrogen) for 1 h.

One microgram of total RNA was reverse transcribed using Superscript reverse transcriptase (Invitrogen).

The amount of SOIG mRNA was expressed as copies per microgram of total RNA.

One microgram of total RNA was reverse transcribed using Superscript II reverse transcriptase (Invitrogen) and random nonamers (Sigma).

One microgram of total RNA was reverse transcribed using M-MLV reverse transcriptase according to the manufacturer's protocol.

One microgram of total RNA was reverse transcribed with avian myeloblastosis virus reverse transcriptase (Promega, USA) and a random hexamer.

One microgram of total RNA was used to synthesize the first strand cDNAs with SuperScript III cDNA Synthesis Kit (Invitrogen).

One microgram of total RNA was treated with the RQ1 RNase-free DNase according to the manufacturer's instructions (Promega).