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Ice cold homogenization buffer (100 mM Tris HCl, 1M NaCl, 4 mM EDTA.Na2, 0.5% Triton X-100) containing 1∶25 freshly made protease inhibitor solution (protease inhibitor cocktail tablet, Roche, USA, 1 tablet dissolved in 1 ml H2O as stock solution) was added quickly to tissues in a volume to weight ratio of 10 µl solution per microgram of tissue.
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Thirty micrograms of tissue proteins were used for the assay.
Here we provide two approaches for the rapid and efficient amplification of amphibian mitogenomes from microgram quantities of tissue.
Methods to amplify complete mitochondrial genomes rapidly and efficiently from microgram quantities of tissue of single individuals are, however, not always available.
Results were reported as micrograms iron/gram of tissue dry weight.
On the other hand, the tumour shows a maximum concentration of Zn-Pc (approximately 0.6 microgram g-1 of tissue) after 18-24 hours; at this time, the ratio between the Zn-Pc levels in the tumour and the muscle (which represents the surrounding normal tissue) is approximately 7.5.
One hundred micrograms of each tissue extract were separated on 12.5% SDS PAGE gel (BioRad) and transferred to PVDF membrane (Pierce).
The yield for G. aurea was ~0.8 micrograms per gram of starting material, 10 fold less that the yield obtained for A. thaliana (~10 micrograms per gram of tissue).
For example, in salmon, the optimal concentration of selenium in the fish tissue (whole body) is about 1 microgram selenium per gram of tissue (dry weight).
Results were expressed as total hydroxyproline content (in micrograms) per 0.1 g of tissue.
The concentrations of these three lGnRHs were detected at levels of pictogram per microgram brain tissue and milliliter of plasma.
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