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Briefly, one microgram of genomic DNA was fragmented by a Covaris device to obtain 350 bp-sized fragments.
Fifteen microgram of genomic DNA was digested with Nco I and separated on a 1% agarose gel.
The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.
The MIAMI method was performed using one microgram of genomic DNA as previously described [16], [17].
One microgram of genomic DNA was digested overnight with either HpaII or MspI (NEB, Ipswich, MA).
Four microgram of genomic DNA was mechanically shared by sonication, and boiled at 95°C for 10 minutes.
Similar(24)
Among the above-mentioned methods, WGBS has been widely used to profile DNA methylation at single-base resolution genome wide; however it consumes micrograms of genomic DNA at a high cost, which is not feasible for many clinical samples, such as tumors obtained by laser capture microdissection or rare stem cell populations [ 16].
Ten micrograms of genomic DNA were used to create next-generation sequencing libraries for exome sequencing.
Three micrograms of genomic DNA were used to generate libraries.
Ten micrograms of genomic DNA at a concentration of 250 ng/µl were used per array.
454-FLX (454 Life Sciences) libraries were generated using three micrograms of genomic DNA.
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