Sentence examples for microdissected sections were from inspiring English sources

All microdissected sections were examined by the CBCP pathologist, who identified and marked regions of tumor before dissection.

Laser microdissected sections were collected in CapSure HS LCM Caps (Molecular Devices, Foster City, CA, USA), and total RNA extraction was performed using PicoPure RNA Isolation Kit (Arcturus, Applied Biosystems) including a treatment with DNase I (RNase-Free DNase Set; Qiagen, Darmstadt, Germany).

Tissue sections were microdissected and DNA and RNA were co-extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen, Hilden, Germany), with an extended overnight digestion for the DNA extraction being the only modification from the manufacturer's instructions.

medium, microdissected and the frozen sections were stained with hematoxylin and eosin (H&E) to check epithelial component.

The samples were embedded in OCT medium, microdissected and the frozen sections were stained with H&E to check epithelial purity.

Tissues were sectioned and sections were further microdissected in order to obtain >80% neoplastic cells.

Tissue sections were manually microdissected before DNA isolation to enrich for tumour cells (>80%).

Briefly, formalin-fixed and paraffin-embedded (FFPE) tissue sections were prepared in 10- μm slices and microdissected to generate samples of tumour and the corresponding normal tissue.

For DNA extraction, the sections were fixed in 70% ethanol, and the number of microdissected cells was ∼10,000 30,000.

To obtain a sufficient number of cells, multiple consecutive sections were immunostained, and ER-negative cell clusters in different sections were microdissected and pooled.

Expressional profiles obtained in 105 samples of whole tissue sections were used to establish oncogenic signaling alterations in progression of CRC, while those representing 40 microdissected specimens were used to select differences in KEGG pathways between epithelium and mucosa.