Sentence examples for microdissected samples were from inspiring English sources

Microdissected samples were collected into a microcentrifuge tube.

Microdissected samples were collected in a tube cap placed below the sample holder.

Microdissected samples were collected into the cap of 0.2-ml microcentrifuge tubes and stored at 4°C.

Microdissected samples were analyzed using direct bisulfite sequencing to determine the DNA methylation status of eight regions within and near the SEPT9 gene.

Microdissected samples were lysed at 56°C for three hours followed by the addition of fresh proteinase K (ProtK) solution, and then overnight incubation at 37°C to ensure complete digestion of the samples.

Data sets from these microdissected samples were analyzed in the following pairs: CEC vs. MUC dissected from distant full-thickness normal colon (NC), normal colon mucosa dissected from tumor (NT), adenoma (AD), and carcinoma (CA).

Quantification of messenger RNA levels from UV laser microdissected samples was carried out by real time quantitative PCR after reverse transcription with a GeneAmp 7900HT (Applied Biosystems).

As primers for D1 and D5 dopamine receptors were not intron-spanning, a DNase digestion step (DNA-free™kit, Ambion®) of UV laser microdissected samples was included before complementary DNA synthesis (Liss and Roeper, 2004).

Total RNA recovered from laser microdissected samples was assessed by measurements of OD260/OD280 and then subjected to two rounds of amplification that generated about 80-100 μg of amplified RNA.

It is also unclear whether the genes identified uniquely using microdissected samples represent useful biomarkers.

While the cancer specimens were not microdissected, these samples were all scored by a pathologist to contain 65 80% tumour tissue.