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Twenty-eight days after transplantation into eight NOD-SCID mice, the microcapsules were explanted.
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The valves were explanted at 3 and 9 months.
The tumors were explanted for DNA preparations.
One liver was explanted and grafted with success.
Healthy ICR mice injected with microcapsules were sacrificed 16 days.
Microcapsules were ground, mixed with KBr and pressed into tabs.
The microcapsules were characterized using various analytical techniques.
After centrifugation, the precipitate containing large microcapsules was discarded, and the functionalized PLGA-coated Fe3O4 microcapsules were generated by a second centrifugation of the remaining microcapsule suspension at 5000 rpm for 5 min.
Microcapsules were first inoculated into liquid media to test the capacity of two selected fungal species, Trichoderma reesei and Aspergillus nidulans, to grow inside the alginate microcapsules.
Fluorescence microscopy demonstrated that PLGA-coated Fe3O4 microcapsules were phagocytosed by MDA cells, with the number of phagocytosed microcapsules increasing with incubation time (12 and 24 h).
Preparation and storage of the microcapsules were performed in the dark.
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