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For BM, only patients with positive microbiological verification (either by blood culture, CSF culture, Gram stain, PCR or agglutination tests) were included [43], [44].
Contrarily, in clinical settings, microbiological verification of an infection and susceptibility of the causative pathogen are usually not available at the time of clinical diagnosis of an infection.
In the present study there were several positive PCR tests with high Cp values and without microbiological verification, and it is difficult to determine their clinical significance.
Initial treatment was defined as the first antibiotic therapy the patient received when submitted to the hospital i.e. prior to the microbiological verification of the bacterial infection.
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Microbiological confirmation was scarce.
In this study, the use of these microbiological parameters for verification of the effect of the stages of washing with pressurized water and air chilling has been evaluated.
A study cohort where all PCR positive samples were supported by a positive BC, or other microbiological or clinical verification, would have been optimal.
We did not have microbiological and radiological verification in all children, so we may not have detected some bacterial infections that spontaneously resolved.
For Hazard Analysis and Critical Control Points (HACCP) verification purposes and compliance with microbiological criteria for foodstuffs, control samplings for Salmonella occur on a two-weekly basis at the slaughterhouse (European Commission, 2005).
Verification was increased when case notes were matched with microbiological or histopathological reports Lack of clarification whether statutory notification and supplemental data sources covered the same catchment area might have compromised the undernotification estimate Ascertainment of cases was limited mainly to hospital patients although patients are treated at other settings.
But trust requires verification".
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