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All isolates were originally identified by standard microbiological techniques in the laboratory of origin.
Although these bacteria could not be cultured by standard microbiological techniques in the present study, this might have been due to the fastidious growth requirements of these organisms, or to the fact that bacteria growing within a surface-associated biofilm displayed viable but non-culturable tendencies.
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Obviously, the two microbiological techniques used in our study may not have been sensitive and specific enough due to properties of the tests themselves [ 20].
Conventional and molecular microbiological techniques were employed in order to determine underlying aetiology.
With the advent of molecular techniques, especially the development of next-generation high-throughput sequencing techniques, more and more bacterial phylotypes, which were undiscovered by traditional microbiological techniques, were found in these habitats of the human body.
In the studies conducted on women with vulvovaginal symptoms, molecular microbiological techniques have been useful in detecting Candida in women who were found to be negative with standard microbiology techniques.
Although recent advances in microbiological techniques (GM analysis, PCR, and so on) showed promising results in identifying IA also in non-conventional subsets of patients, such as the critically ill, a high level of suspicion of IA should be maintained especially when risk factors (for example, COPD, steroid use) are present.
In conclusion, detection of Mycobacteria either by microscopy/classical microbiological techniques or by DNA amplification techniques in pleural effusion patients was enhanced by examining pleural fluid as well as sputum collected in tandem.
A clinical isolate of a methicillin-resistant S. aureus (MRSA 27065) recovered from a patient with an infected total hip arthroplasty and an isolate from an infected central venous line [S. epidermidis SE 183 (Gollwitzer et al. 2003)] were identified by standard microbiological techniques and also applied in the growth inhibition assays.
The media as well as the genetic and microbiological techniques were essentially as in Rose et al. [11].
With this in mind, if less than one percent of microbial populations can be cultured using standard microbiological techniques due to incongruencies in direct counts versus cultivatable microbes [29] [32], then only a very small number of viruses have the likelihood of being genomically described.
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