The species identification of the isolates was based on standard microbiological tests: colony morphology, acid-fast staining, and biochemical tests [ 6].
Characteristic colonies of S.aureus (round, convex, golden yellow, mannitol fermenting colonies on MSA plates) were aseptically picked, further purified by repeated streaking on Nutrient agar plate and characterized following established microbiological methods (evaluation for colonial morphology, cell shape and grouping, Gram reaction, catalase and coagulase tests).
The identity of the presumptive Enterobacteriaceae isolates was confirmed by conventional microbiological methods, including colony and cellular morphology and Gram staining.
307 bacterial isolates were classified based on morphology, Gram-staining and standard microbiological tests.
After getting pure colonies, further identification were conducted using standard microbiological techniques, which include Gram stain, colony morphology and biochemical tests.
Bacterial isolates were identified using standard microbiological procedures, as growth and colonial characteristics, Gram staining, cellular morphology, catalase and oxidase reactions, coagulase test (Slidex Staph Plus, bioMérieux, Marcy-l'Etoile, France), haemolysin production.
Blood and CSF were processed by standard microbiological methods (3 ). S. pneumoniae isolates were identified by colony morphology and α-hemolysis and then confirmed by Gram staining and determination of optochin susceptibility (Oxoid, Basingstoke, UK).
Microbiological differentiation of H. influenzae from other species has conventionally relied upon colonial morphology, haemin and NAD (X and V factor) dependence, and for capsular strains, serotyping using various methods [ 15].
